Plant imaging: advanced light microscopy - a joint course with CUSO MPS
1-2 february 2024
Venue: University of Geneva, building Sciences III, Room 0059
Instructors: Dr. Sylvain Loubéry (University of Geneva) and Dr. Christoph Bauer (University of Geneva)
Description
The course will focus on light microscopy, and the objective of the course is two-fold:
- to acquire or strengthen basic knowledge of the most common methods of plant optical imaging (including sample preparation and staining);
- to acquire or strengthen basic skills in image acquisition and in image analysis.
The course includes three lectures on basic and advanced fluorescence microscopy, sample preparation, and image analysis.
In parallel, hands-on sessions will be held on fluorescence microscopy and on image analysis.
During the hands-on sessions, all participants will use at least three different set-ups, including widefield as well as CLSM confocal microscopes. These sessions will be done with samples brought by the participants. For the hands-on microscopy parts, participants will be grouped by 3 and the groups will rotate between different microscopes. During the image analysis hands-on, participants will work on image analysis and Fiji; this will be done on laptops brought by the participants.
The practicals will take place at the Bioimaging Center of UniGE.
The participants are expected to have prior experience with microscopy (and preferably with confocal microscopy).
Participants will be asked to bring their own laptops for the image analysis hand-on session.
Program
Find detailed program here
Flyer here
Thursday, February 1 st
9h-10h: Registration Introduction Participants’ presentations Room 0059 (Sciences III)
10h-11h45: Lecture “Fluorescence microscopy” Room 0059 (Sciences III)
11h45-13h: Lunch break
13h -14h30: Hands-on microscopy (1st rotation) Bioimaging Center (Sciences II)
14h30-14h45: Coffee break
14h45-16h30: Room 0059 Lecture “Sample preparation” (Sciences III)
Friday, February 2nd
9h -10h45: Hands-on “Intro to histology” Lab 2065A (Sciences III)
10h45- 11h: Coffee break
11h – 12h30: Hands-on microscopy (2nd rotation) Bioimaging Center (Sciences II)
12h30-13h30: Lunch break
13h30 -15h: Hands-on microscopy (3rd rotation) Bioimaging Center (Sciences II)
15h-17h:Hands-on image analysis Room 0059 (Sciences III)
General information
Travel expenses covered by DSLS (return ticket to Geneva on the basis of a 2nd class half-price ticket)
Make sure you sign the attendance sheet each and every day.
Registration
Registration here
Maximum number of participants: 9 people (minimum 6).
4 places available for DSLS students.
A waiting list will be prepared to ensure that there are no empty seats.
Deadline for registration: 22.01.2024
*For cancellations after the deadline of registration or no-show: 50 CHF administrative fee
Credits
Credits: 1.0
Category: Research tools (R)