My research is focus on terrestrial eukaryotes biogeography and macroecology.

My main project focus on island biogeography along elevation gradients, and aim to understand the main drivers of protists island endemicity and phylogeography. This project is funded by the SNF (project number 182531).

In a second project funded by the Swiss FOEN, I am developing new molecular techniques to characterize the diversity of springtails and mites in Switzerland. This work is done in collaboations with horizon a.

My last project aims at disclosing potnetial effect of warming on the soil microbial loop, particularly on heterotrophic protist impact on nutrioent cycles in forest soils.

I also develop bioinformatic tools for the analyses of eDNA HTS datasets for metabarcode based diversity assessments, tools for in-silico PCR, barcode raw data analyses, network analyses (see on github).

 

Research Project

Biodiversity and biogeography of soil protists in continental and oceanic islands - SNSF funded to Prof. Edward Mitchell - 2019-2023

Inventaire national des collemboles et acariens et développement du metabarcoding pour le monitoring de la biodiversité des sols - OFEV funded to Prof. Edward Mitchell, Dr. Guillaume Lentendu, Sylvain Lanz (horizon a) and Loïc Bulliard (horizon a) - 2021-2024

Heterotrophic protists impact on forest soil prokaryotes and nutrients cycles in a global warming context - Bois Chamblard Foundation funded to Dr. Guillaume Lentendu - 2023-2025

 

Education history

2004 - 2006

B.Sc. in Biology, Paul Verlaine University, Metz, France

2006 – 2007

B.Sc. (third year) in Biology of Organism, Population and Ecosystem, Paul Sabatier University, Toulouse, France

2007 - 2009

M.Sc. in Ecology, Paul Sabatier University, Toulouse, France

2010 - 2014

PhD in Microbial Ecology, University of Leipzig and UFZ Halle (Saale), Germany

 

Employment history

2013 -2014

Research assistant in bioinformatic, Helmholtz Centre for Environmental Research-UFZ Halle (Saale), Germany

2014 - 2016

PostDoc in the GFBio project, Helmholtz Centre for Environmental Research-UFZ Halle (Saale), Germany

2016 - 2019

PostDoc in protist biogeography and macroecology, University of Kaiserslautern, Germany

2019 - present

PostDoc in protist and testate amoebae biogeography, University of Neuchâtel, Switzwerland

 

Publications

2023

Lentendu, G., Bruni, E., Ah-Peng, C., Fujinuma, J., Kubota, Y., Lorite, ... Mitchell, E.A.D. Soil filtration-sedimentation improves shelled protist recovery in eukaryotic eDNA surveys. Molecular Ecology Ressources, 23(6), 1361–1371

Lentendu, G., Lara, E., & Geisen, S. Metabarcoding Approaches for Soil Eukaryotes, Protists, and Microfauna. In F. Martin & S. Uroz (Eds.), Microbial Environmental Genomics (MEG) (pp. 1–16). Springer US.

2022

Gulin Beljak, V., Kulaš, A., Lentendu, G., Vlaičević, B., Gligora Udovič, M., Sertić Perić, ... Matoničkin Kepčija, R. Changes in Phylogenetic and Functional Diversity of Ciliates along the Course of a Mediterranean Karstic River. Microorganisms, 10(12), Article 12.

Jung, P., Lehnert, L. W., Bendix, J., Lentendu, G., Grube, M., Alfaro, F. D., ... Lakatos, M. The grit crust: A poly-extremotolerant microbial community from the Atacama Desert as a model for astrobiology. Frontiers in Astronomy and Space Sciences, 9.

Lansac-Tôha, F. M., Buosi, P. R. B., ... , Dunthorn, M., Lentendu, G., Lansac-Tôha, F. A., & Velho, L. F. M. Molecular insight reveals broad-scale spatial patterns in floodplain ciliate communities, while morphology reflects local environmental controls. Freshwater Science, 41(2).

2021

Francioli, D., Lentendu, G., Lewin, S., & Kolb, S. DNA Metabarcoding for the Characterization of Terrestrial Microbiota—Pitfalls and Solutions. Microorganisms, 9(2), 361.

Kulaš, A., Gulin, V., Matoničkin Kepčija, R., Žutinić, P., Sertić Perić, M., Orlić, S., Kajan, K., Stoeck, T., Lentendu, G., Čanjevac, I., Martinić, I., & Gligora Udovič, M. (2021). Ciliates (Alveolata, Ciliophora) as bioindicators of environmental pressure: A karstic river case. Ecological Indicators, 124, 107430.

Lentendu, G., & Dunthorn, M. Phylogenetic relatedness drives protist assembly in marine and terrestrial environments. Global Ecology and Biogeography, 30(7), 1532–1544.

Singer, D., Seppey, C. V. W., Lentendu, G., Dunthorn, M., Bass, D., Belbahri, L., .... Lara, E. Protist taxonomic and functional diversity in soil, freshwater and marine ecosystems. Environment International, 146, 106262.

2020

Forster, D., Lentendu, G., Wilson, M., Mahé, F., Leese, F., Andersen, T., Khomich, M., & Dunthorn, M. Evaluating geographic variation within molecular operational taxonomic units (OTUs) using network analyses in Scandinavian lakes. BioRxiv, 2020.08.06.240267.

Frühe, L., Cordier, T., Dully, V., Breiner, H.-W., Lentendu, G., Pawlowski, J., Martins, C., Wilding, T. A., & Stoeck, T. Supervised machine learning is superior to indicator value inference in monitoring the environmental impacts of salmon aquaculture using eDNA metabarcodes. Molecular Ecology, 30(13), 2988–3006.

Theodorou, P., Radzevičiūtė, R., Lentendu, G., Kahnt, B., Husemann, M., Bleidorn, C., ... Paxton, R. J. Urban areas as hotspots for bees and pollination but not a panacea for all insects. Nature Communications, 11(1), 1–13.

2019

Lentendu, G., Buosi, P. R. B., Cabral, A. F., Segovia, B. T., Meira, B. R. de, Lansac-Toha, F. M., … Dunthorn, M., Protist Biodiversity and Biogeography in Lakes From Four Brazilian River–Floodplain Systems. Journal of Eukaryotic Microbiology, 66(4), 592-599

Forster, D., Lentendu, G., Filker, S., Dubois, E., Wilding, T.A., Stoeck, T.,, Improving eDNA-based protist diversity assessments using networks of amplicon sequence variants. Environmental Microbiology

2018

Lentendu, G., Mahé, F., Bass, D., Rueckert, S., Stoeck, T., & Dunthorn, M., Consistent patterns of high alpha and low beta diversity in tropical parasitic and free-living protists. Molecular Ecology, 27(13), 2846–2857

Banos, S., Lentendu, G., Kopf, A., Wubet, T., Glöckner, F. O., & Reich, M., A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms. BMC Microbiology, 18(1), 190

Purahong, W., Wubet, T., Kahl, T., Arnstadt, T., Hoppe, B., Lentendu, G., … Buscot, F., Increasing N deposition impacts neither diversity nor functions of deadwood-inhabiting fungal communities, but adaptation and functional redundancy warrants ecosystem function. Environmental Microbiology, 20(5), 1693–1710

Purahong, W., Wubet, T., Lentendu, G., Hoppe, B., Jariyavidyanont, K., Arnstadt, T., … Buscot, F., Determinants of deadwood-inhabiting fungal communities in temperate forests: molecular evidence from a large scale deadwood decomposition experiment. Frontiers in Microbiology, 9

Schöps, R., Goldmann, K., Herz, K., Lentendu, G., Schöning, I., Bruelheide, H., … Buscot, F., Land-Use Intensity Rather Than Plant Functional Identity Shapes Bacterial and Fungal Rhizosphere Communities. Frontiers in Microbiology, 9.

Weißbecker, C., Wubet, T., Lentendu, G., Kühn, P., Scholten, T., Bruelheide, H., & Buscot, F., Experimental Evidence of Functional Group-Dependent Effects of Tree Diversity on Soil Fungi in Subtropical Forests. Frontiers in Microbiology, 9.

2017

Mahé, F., de Vargas, C., Bass, D., Czech, L., Stamatakis, A., Lara, E., ... Lentendu, G., ... Dunthorn, M., Parasites dominate hyperdiverse soil protist communities in Neotropical rainforests. Nature Ecology & Evolution 1, 0091.

Morawe, M., Hoeke, H., Wissenbach, D. K., Lentendu, G., Wubet, T., Kröber, E., & Kolb, S., Acidotolerant Bacteria and Fungi as a Sink of Methanol-Derived Carbon in a Deciduous Forest Soil. Frontiers in Microbiology, 8.

Purahong, W., Pietsch, K., Lentendu, G., Schöps, R., Bruelheide, H., Wirth, C., … Wubet, T. et al., Characterization of unexplored deadwood mycobiome in highly diverse subtropical forests using culture-independent molecular technique. Frontiers in Microbiology 8: 574.

Francioli, D., Schulz, E., Lentendu G., Wubet, T., Buscot, F., & Reitz, T., Mineral vs. Organic Amendments: Microbial Community Structure, Activity and Abundance of Agriculturally Relevant Microbes Are Driven by Long-Term Fertilization Strategies. Frontiers in Microbiology, 7, 1446.

2016

Purahong W, Wubet T, Lentendu G., … Buscot D., Life in leaf litter: novel insights into community dynamics of bacteria and fungi during litter decomposition. Molecular Ecology 25 (16), 4059-4074

2015

Ramirez K. S., Döring M., Eisenhauer N., Gardi C., Ladau J., Leff J. W., Lentendu G., ... Wall D. H. (2015) Towards a global platform for linking soil biodiversity data, Frontiers in Ecology and Evolution, 3: 91

Purahong W., Stempfhuber B., Lentendu G., Francioli D., Reitz T., Buscot F., Schloter M., Krüger D. (2015) Influence of Commonly Used Primer Systems on Automated Ribosomal Intergenic Spacer Analysis of Bacterial Communities in Environmental Samples, PLoS One, doi: 10.1371/journal.pone.0118967

2014

Lentendu G., Chatzinotas A., Wubet T., Buscot F., Wilhelm C., Schlegel M. (2014) Effects of long term differential fertilization on eukaryotic microbial community of an arable soil: a multiple barcoding approach. Molecular Ecology, 23(13): 3341-3355

2013

Lentendu G., Hübschmann T., Müller S., Dunker S., Buscot F., Wilhelm C. (2013) Recovery of soil unicellular eukaryotes: an efficiency and activity analysis on the single cell level. Journal of Microbiological Methods, 95: 463-469

2011

Lentendu G., Zinger L., Manel S., Coissac E., Choler P., Geremia R., Melodelima C. (2011) Assessment of soil fungal diversity in different alpine tundra habitats by means of pyrosequencing. Fungal Diversity, 49(1): 113-123

 

Review for

Acta Oecologia, Applied Soil Ecology, Biology & Fertility of Soil, Cytometry Part A, Environmental Microbiology Reports, FEMS Microbiology Ecology, Microbial Ecology, Molecular Ecology, Molecular Ecology Resources, Research in Microbiology, Revista Ambiente & Água, Science of The Total Environment, Scientific Reports, Systematics and Biodiversity

Teaching

2019/20

Bioinformatics and statistics for metabarcoding studies in environmental microbiology and ecology, practical course, joint course of CUSO Doctoral Program in Ecology and Evolution and Interuniversity Doctoral Program in Organismal Biology, University of Neuchâtel, Switzerland (16h)

2018/19

Introducing DeltaMP, an automatic metabarcoding bioinformatic pipeline for HPC, practical course, Ecology Dept., University of Kaiserslautern, Germany (8 h)

Bioinformatic and statistics in microbial community ecology, master Ecology and Microbial Biodiversity, practical course, University of Kaiserslautern, Allemagne (16 h)

2017/18

Ecology and diversity of aquatic microbial eukaryotes, practical course, master Ecology and Microbial Biodiversity, University of Kaiserslautern, Allemagne (16 h)

Bioinformatic and statistics in microbial community ecology, lecture, master Ecology and Microbial Biodiversity, University of Kaiserslautern, Allemagne (16 h)

2016/17

Bioinformatic and statistics in microbial community ecology, practical course, master Ecology and Microbial Biodiversity, University of Kaiserslautern, Allemagne (16 h)

2015/16

Assessing microbial diversity and interactions in soil with NGS, practical course, master Evolution and Ecology, University of Leipzig, Germany (4 h)

Submission of raw amplicon sequences and their associated metadata to Short Read Archives databases, practical course, HIGRADE graduate school, UFZ Halle/Salle, Allemagne (8 h)

2011/12

Introduction to unix bash language, OBITools and virtual In silico PCR, practical course, HIGRADE graduate school, UFZ Halle/Salle, Allemagne (8 h)

 

Student co-supervision

2019- present

E. Bruni, PhD thesis, Biodiversity And Biogeography of Euglyphida (Rhizaria) in Continental and Oceanic Islands, University of Neuchâtel

2018/19

I. Simeth, B.Sc., Einfluss der Artensubstitution und Änderungen des Artenreichtums auf marine Protisten entlang des LDG, University of Kaiserslautern

2017/18

V. Immel, B.Sc., Parametric vs. Non-parametric estimation of Neotropical soil protist diversity, University of Kaiserslautern

V. Dully, M.Sc., Inferring Indicator Values from ciliate eDNA metabarcodes for ecological status assessment of salmon farms, University of Kaiserslautern

M. Wilson, B.Sc., Protist intra-OTU biogeographic patterns in Norvegian Lakes, University of Kaiserslautern

2016/2017

N. Faulhaber, M.Sc., Latitudinal Diversity Gradient of protistan plankton, University of Kaiserslautern

2015/16

C. Porges, M.Sc., Changes in rhizosphere microbial community association with different parental types of Arabidospsis thaliana MAGIC lines, University of Leipzig

2012

S. Gerstl, B.Sc., Response of soil ciliates diversity to fertilization, University of Leipzig

 

Invited talks

2019

Metabarcoding soil protists for macroecology studies: insight into bioinformatic and use cases, Lab. Biodiversity & Conservation Biogeography , University of the Ryukyus, Okiinawa, Japan, September 4^th

2016

Microbial diversity and interactions in cultivated soils, Ecology Dept. seminar, University of Kaiserslautern, Germany, January 16^th

2015

High-throughput single cell and molecular methods to analyse soil eukaryotic microbes and their long term changes in response to soil fertilisation, Ecological Symposium, University of Cologne, Germany, February 14^th

2014

Metagenomics and amplicon sequencing for species and phylogenetic diversity assessment of complex communities, workshop iSEQ, iDiv Leipzig, Germany, Novembre  13^th to 14^th

2013

Effect of long term differential fertilization on the soil microbial eukaryotic community, Laboratoire Ampère, Ecole Centrale de Lyon, France, Octobre 14^th

Effect of long term differential fertilization on the soil microbial eukaryotic community, Molecular Systématic group seminar, université de Genève, Switzerland, Octobre 15^th

 

Talks

2019

Biogeography of protists in Neotropical rainforest soils and in the oceans, workshop Protists diversity and ecology in aquatic and terrestrial ecosystems, University of Neuchâtel, Swotzerland

2018

Latitudinal diversity gradient of protistan plankton in the Atlantic ocean, The 5th joint meeting of the Phycological Society of America & International Society of Protistologists, Vancouver, Canada

Phylogenetically Close Protists Co-occur: Assembly Patterns in Two Large Scale Environmental Sequencing Surveys, MACRO 2018, Birmensdorf, Switzerland

2017

Persistent biogeographic patterns of tropical free-living and parasitic protists, 15th international congress of protistology, Prague, Czech Republic

Persistent patterns of protist biogeography in three Neotropical rainforest, 37th annual meeting of the German Society for Protozoology, Meißen, Germany

2014

Effects of long term differential fertilization on microbial eukaryotic community of an arable soil: a multiple barcoding approach, 33rd annual meeting of the German Society for Protozoology, Essen, Germany

2013

Soil eukaryotic unicellular microorganisms facing crop fertilization: a metagenomic approach, Annual Conference of the Association for General and Applied Microbiology (VAAM), Bremen, Germany

Soil eukaryotic unicellular microorganisms facing crop fertilization, BioSyst.EU 2013 Global systematics!, Vienna, Austria

2011

High-throughput analysis of soil eukaryotic unicellular microorganisms: from cytometry to pyrosequencing, 5th International Conference on Analysis of Microbial Cells at the Single Cell Level, Marseille, France

 

Travel grants

2018

Holz-Conner award from the International Society of Protozoology to Vancouver, Canada

2017

from the International Research Coordination Network for Biodiversity of Ciliates to Meißen, Germany

Holz-Conner award from ISOP to Prague, Czech Republic

2016

from the International Research Coordination Network for Biodiversity of Ciliates to Lyon, Fance

 

Posters

2016

Lentendu G., Mahé F., Dunthorn M., Biogeography of predatory protists in Neotropical forest soils, 17ème Journées Ouvertes en Biologie, Informatique et Mathématiques (JOBIM), Lyon, France

2015

Lentendu G., Schmidt J., Schultz E., Buscot F., Wubet T., Alfalfa root symbionts under soil nutrient pressure: cooperation or competition?, Ecology Of Soil Microorganism, Prague, Czech Republic

Lentendu G., Schmidt J., Schultz E., Buscot F., Wubet T., Alfalfa root symbionts under soil nutrient pressure: cooperation or competition?, 13th Symposium on Bacterial Genetics and Ecology, Milan, Italy

2014

Lentendu G., Schmidt J., Schultz E., Buscot F., Wubet T.,"How do crop fertilization affect the soil microbiome on a long-term basis?", The First Global Soil Biodiversity Conference, Dijon, France

2013

Lentendu G., Wubet T., Chatzinotas A., Wilhelm C., Schlegel M., Buscot F.,Effect of long term differential fertilization on the soil microbial eukaryotic community, 2nd Thünen Symposium on Soil Metagenomics, Brunswick, Allemagne

Lentendu G., Wubet T., Hübschmann T., Müller S., Schlegel M., Wilhelm C., Buscot F.,Soil unicellular eukaryotic community analyzed at the single cell level: coupling flow cytometry and metagenomics, the 5th Congress of European Microbiologist, Leipzig, Allemagne

2011

Lentendu G., Müller S., Hübschmann T., Wubet T., Buscot F., Schlegel M., Wilhelm C.,"High-throughput analysis of soil eukaryotic unicellular microorganisms: from cytometry to pyrosequencing", Workshop of the GfÖ Soil Ecology Group, Berlin, Allemagne