Jean-Marc Neuhaus

Curriculum vitae

Naissance à Bienne (Suisse) en 1955

Marié, deux enfants

Ecoles à Bienne

1973-1976: Etudes de biologie II à Bâle (Biozentrum de l'université), diplôme en biophysique

1977-1982: Thèse de doctorat en biochimie (directeur de thèse Jörg Rosenbusch) sur la maltoporine d'E.coli

1982-1984: post-doctorat à l'Institut Max-Planck de Biologie de Tübingen (Allemagne) dans le laboratoire du Prof. Peter Overath sur la perméase de lactose d'E. coli.

1984-1988: post-doctorat à l'Institut Friedrich-Miescher de Bâle (Suisse) dans le laboratoire du Prof. Fred Meins, Jr. sur le rôle des chitinases du tabac dans la défense contre les pathogènes

1988-1995: post-doctorat, puis assistanat à l'Institut de Botanique, département de physiologie végétale, de l'Université de Bâle dans le laboratoire du Prof. Thomas Boller sur le traffic intracellulaire des protéines vacuolaires

1995-présent: professeur de biochimie et biologie moléculaire à l'Institut de Botanique de l'Université de Neuchâtel

1999-2007: Membre du Comité Central de l'Académie Suisse des Sciences Naturelles (scnat)

2001-2005: directeur de l'Institut de Botanique

depuis 2002: vice-directeur du Pôle de Recherche National (NCCR) "Survie des plantes"

depuis 2010: vice-directeur de l'Institut de Biologie de l'Université de Neuchâtel

depuis 2010: président de la Commission Suisse pour la Biologie Moléculaire (SKMB)



  • Shen, Y., Wang, J., Ding, Y., Lo, S.W.,  Gouzerh,G., Neuhaus, J.-M. and Jiang, L. ( 2011 )The Rice RMR1 Associates with a Distinct Prevacuolar Compartment for the Protein Storage Vacuole Pathway. Molecular Plant ( in press )
  • Sriranganadane, D., Reichard, U., Salamin, K., Fratti, M., Jousson, O., Waridel, P., Quadroni, M., Neuhaus, J.-M., and Monod, M. (2011). Secreted glutamic protease rescues aspartic protease Pep deficiency in Aspergillus fumigatus during growth in acidic protein medium. Microbiology (accepted)
  • Legay G, Marouf E, Berger D, Neuhaus J-M, Mauch-Mani B, Slaughter A ( 2011 ) Identification of genes expressed during the compatible interaction of grapevine with Plasmopara viticola through suppression subtractive hybridization (SSH). European J. Plant Pathol. 129, 281-301
  • Sriranganadane, D., Waridel, P., Salamin, K., Reichard, U., Grouzmann, E., Neuhaus, J.M., Quadroni, M., and Monod, M. (2010). Aspergillus protein degradation pathways with different secreted protease sets at neutral and acidic pH. J Proteome Res 9, 3511-3519.
  • Schaefer, D.G., Delacote, F., Charlot, F., Vrielynck, N., Guyon-Debast, A., Le Guin, S., Neuhaus, J.M., Doutriaux, M.P., and Nogue, F. (2010). RAD51 loss of function abolishes gene targeting and de-represses illegitimate integration in the moss Physcomitrella patens. DNA Repair (Amst) 9, 526-533.
  • Saint-Jean, B., Seveno-Carpentier, E., Alcon, C., Neuhaus, J.-M., and Paris, N. (2010). The Cytosolic Tail Dipeptide Ile-Met of the Pea Receptor BP80 Is Required for Recycling from the Prevacuole and for Endocytosis. Plant Cell.
  • Paris, N., Saint-Jean, B., Faraco, M., Krzeszowiec, W., Dalessandro, G., Neuhaus, J.M., and Di Sansebastiano, G.P. (2010). Expression of a glycosylated GFP as a bivalent reporter in exocytosis. Plant Cell Rep 29, 79-86.
  • Slaughter, A., Hamiduzzaman, M., Gindro, K., Neuhaus, J.-M., and Mauch-Mani, B. (2008). Beta-aminobutyric acid-induced resistance in grapevine against downy mildew: involvement of pterostilbene. European Journal of Plant Pathology 122, 185-195.
  • Humbert, N., Schurmann, P., Zocchi, A., Neuhaus, J.M., and Ward, T.R. (2008). High-yield production and purification of recombinant T7-tag mature streptavidin in glucose-stressed E. coli. Methods Mol Biol 418, 101-110.
  • Frutos, R., Denise, H., Vivares, C., Neuhaus, J.M., Vitale, S., Pedrazzini, E., Ma, J., Dix, P., Gray, J., Pezzotti, M., Conrad, U., and Robinson, D. (2008). Pharmaceutical proteins in plants. A strategic genetic engineering approach for the production of tuberculosis antigens. Ann N Y Acad Sci 1149, 275-280.
  • Finka, A., Saidi, Y., Goloubinoff, P., Neuhaus, J.M., Zryd, J.P., and Schaefer, D.G. (2008). The knock-out of ARP3a gene affects F-actin cytoskeleton organization altering cellular tip growth, morphology and development in moss Physcomitrella patens. Cell Motil Cytoskeleton 65, 769-784.
  • Di Sansebastiano, G.P., Rehman, R.U., and Neuhaus, J.-M. (2007). Rat β-glucuronidase as a reporter protein for the analysis of the plant secretory pathway. Plant Biosystems 141, 329-336.
  • Saint-Jore-Dupas, C., Gilbert, M.-A., Ramis, C., Paris, N., Kiefer-Meyer, M.-C., Neuhaus, J.-M., Faye, L., and Gomord, V. (2005). Targeting of proConA to the Plant Vacuole Depends on Its Nine Amino-Acid C-Terminal Propeptide. Plant Cell Physiol 46, 1603-1616.
  • Neuhaus, J.-M., and Paris, N. (2005). Plant vacuoles. from biogenesis to function. In Plant Endocytosis, J. Samaj, F. Baluska, and D. Menzel, eds, pp. 63-82.
  • Hernandez, D.H., Paris, N., Neuhaus, J.M., and Deloche, O. (2005). The Yeast Saccharomyces cerevisiae Is Not an Efficient Tool for in Vivo Studies of Plant Vacuolar Sorting Receptors. Plant Cell 17, 1339-1342.
  • Hamiduzzaman, M.M., Jakab, G., Barnavon, L., Neuhaus, J.-M., and Mauch-Mani, B. (2005). beta-Aminobutyric acid-induced resistance against downy mildew in grapevine acts through the potentiation of callose formation and jasmonic acid signaling. Mol Plant Microbe Interact 18, 819-829.
  • Grallath, S., Weimar, T., Meyer, A., Gumy, C., Suter-Grotemeyer, M., Neuhaus, J.M., and Rentsch, D. (2005). The AtProT family. Compatible solute transporters with similar substrate specificity but differential expression patterns. Plant Physiol. 137, 117-126.
  • Happel, N., Höning, S., Neuhaus, J.-M., Paris, N., Robinson, D.G., and Holstein, S.E. (2004). Arabidopsis mu A-adaptin interacts with the tyrosine motif of the vacuolar sorting receptor VSR-PS1. Plant J. 37, 678-693.
  • Zocchi, A., Jobe, A.M., Neuhaus, J.-M., and Ward, T.R. (2003). Expression and purification of a recombinant avidin with a lowered isoelectric point in Pichia pastoris. Protein Expr Purif 32, 167-174.
  • Flückiger, R., De Caroli, M., Piro, G., Dalessandro, G., Neuhaus, J.M., and Di Sansebastiano, G.P. (2003). Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins. J. Exp. Bot. 54, 1577-1584.
  • Paris, N., and Neuhaus, J.M. (2002). BP-80 as a vacuolar sorting receptor. Plant Mol. Biol. 50, 903-914.
  • Ceriotti, A., Paris, N., Hillmer, S., Frigerio, L., Neuhaus, J.-M., Vitale, A., and Robinson, D.G. (2002). Plant cell biology. In Essential cell biology, M. Lord and J. Davey, eds (Oxford: Oxford University Press.
  • Brandizzi, F., Frangne, N., Marc-Martin, S., Hawes, C., Neuhaus, J.M., and Paris, N. (2002). The destination for single-pass membrane proteins is influenced markedly by the length of the hydrophobic domain. Plant Cell 14, 1077-1092.
  • Neuhaus, J.-M., and Boevink, P. (2001). The green fluorescent protein (GFP) as a reporter in plant cells. In Plant Cell Biology, C.R. Hawes and B. Satiat-Jeunemaitre, eds (Oxford: Oxford University Press), pp. 127-142.
  • Di Sansebastiano, G.P., Paris, N., Marc-Martin, S., and Neuhaus, J.-M. (2001). Regeneration of a lytic central vacuole and of neutral peripheral vacuoles can be visualized by green fluorescent proteins targeted to either type of vacuoles. Plant Physiol. 126, 78-86.
  • Neuhaus, J.-M. (2000). GFP as a marker for vacuoles in plants. In Vacuolar Compartments, D.G. Robinson and J.C. Rogers, eds (Sheffield: Sheffield Academic Press Ltd.
  • Neuhaus, J.-M., and Martinoia, E. (1999). Plant cell vacuoles. In Encyclopedia of Life Sciences (Basingstoke: Macmillan Publishers.
  • Neuhaus, J.-M. (1999). Plant chitinases (PR-3, PR-4, PR-8, PR-11). In Pathogenesis-related proteins in plants, S.K. Datta and S. Muthukrishnan, eds (Cambridge: CRC Press), pp. 77-105.
  • Matsuoka, K., and Neuhaus, J.-M. (1999). Cis-elements of protein transport to the plant vacuoles. Journal of Experimental Botany 50, 165-174.
  • Grieco, F., Castellano, M.A., Di Sansebastiano, G.P., Maggipinto, G., Neuhaus, J.-M., and Martelli, G. (1999). Subcellular localization and in vivo identification of olive latent virus 2 putative movement protein. Journal of General Virology 80, 1103-1109.
  • Schlumbaum, A., Jacomet, S., and Neuhaus, J.-M. (1998). Coexistence of tetraploid and hexaploid naked wheat in a neolithic lake dwelling of Central Europe: Evidence from morphology and ancient DNA. Journal of Archaeological Science 25, 1111-1118.
  • Neuhaus, J.M., and Rogers, J.C. (1998). Sorting of proteins to vacuoles in plant cells. Plant Mol Biol 38, 127-144.
  • Iseli-Gamboni, B., Boller, T., and Neuhaus, J.-M. (1998). Mutation of either of two essential glutamates converts the catalytic domain of tobacco class I chitinase into a chitin-binding lectin. Plant Science 134, 45-51.
  • Di Sansebastiano, G.P., Paris, N., Marc-Martin, S., and Neuhaus, J.-M. (1998). Specific accumulation of GFP in a non-acidic vacuolar compartment via a C-terminal propeptide-mediated sorting pathway. The Plant Journal 15, 449-457.
  • Münch-Garthoff, S., Neuhaus, J.-M., Boller, T., Kemmerling, B., and Kogel, K.-H. (1997). Expression of β-1,3-glucanase and chitinase in healthy, stem rust-affected and elicitor-treated near-isogenic wheat lines showing Sr5 or Sr24-specified race-specific rust resistance. Planta 201, 235-244.
  • Neuhaus, J.-M., Fritig, B., Linthorst, H.J.M., Meins, F., Jr., Mikkelsen, J.D., and Ryals, J. (1996). A revised nomenclature for chitinase genes. Plant Molecular Biology Reporter 14, 102-104.
  • Neuhaus, J.-M. (1996). Protein targeting to the plant vacuole. Plant Physiology and Biochemistry 34, 102-104.
  • Lan, V.T.T., Neuhaus, J.-M., Boller, T., Broughton, W.J., and Krause, A. (1996). Molecular cloning of Vigna cDNAs encoding class I (Accession No. X88800) and class IV (Accession No. X88803). Plant Physiology 110, 335.
  • Iseli, B., Boller, T., and Neuhaus, J.-M. (1996). Functional analysis of the domains of a plant PR-3 chitinase. In Chitin Enzymology, R.A.A. Muzzarelli, ed (Grottamare: Atec Edizioni), pp. 135-142.
  • Iseli, B., Armand, S., Boller, T., Neuhaus, J.-M., and Henrissat, B. (1996). Plant chitinases use two different hydrolytic mechanisms. Federation of European Biochemical Societies Letters 382, 186-188.
  • Neuhaus, J.M., Pietrzak, M., Freydl, E., and Boller, T. (1995). Expression and Correct Localization of Animal Secretory Proteins in Plant-Cells. Journal of Cellular Biochemistry, 146-146.
  • Freydl, E., Meins, F.J., Boller, T., and Neuhaus, J.-M. (1995). Kinetics of prolyl hydroxylation, intracellular transport and C-terminal processing of the tobacco vacuolar chitinase. Planta 147, 250-256.
  • Neuhaus, J.-M., Pietrzak, M., and Boller, T. (1994). Mutation analysis of the C-terminal vacuolar targeting peptide of tobacco chitinase: low  specificity of the sorting system, and gradual transition between intracellular retention and secretion into the extracellular space. Plant Journal 5, 45-54.
  • Meins, F., Fritig, B., Linthorst, H.J.M., Mikkelsen, J.D., Neuhaus, J.-M., and Ryals, J. (1994). Plant chitinase genes. Plant Molecular Biology Reporter 12, S22-S28.
  • Vierheilig, H., Alt, M., Neuhaus, J.-M., Boller, T., and Wiemken, A. (1993). Effect of chitinase overexpression on the colonization of roots by pathogenic and symbiotic fungi. Molecular Plant-Microbe Interactions 6, 261-264.
  • Sticher, L., Hofsteenge, J., Neuhaus, J.-M., Boller, T., and Meins, F. (1993). Posttranslational processing of a new class of hydroxyproline-containing proteins - prolyl hydroxylation and C-terminal cleavage of tobacco (Nicotiana tabacum) vacuolar chitinase. Plant Physiol 101, 1239-1247.
  • Neuhaus, J.M. (1993). Estimation efficiency and tests of covariate effects with clustered binary data. Biometrics 49, 989-996.
  • Iseli, B., Boller, T., and Neuhaus, J.-M. (1993). The N-terminal cysteine-rich domain of tobacco class I chitinase is essential for chitin binding but not for catalytic or antifungal activity. Plant Physiology 103, 221-226.
  • De Virgilio, C., Burckert, N., Neuhaus, J.-M., Boller, T., and Wiemken, A. (1993). CNE1, a Saccharomyces cerevisiae homologue of the genes encoding mammalian calnexin and calreticulin. Yeast 9, 185-188.
  • Beffa, R., Neuhaus, J.-M., and Meins, F., Jr. (1993). Physiological compensation in antisense transformants: Specific induction of an "ersatz" glucan endo-1,3-β-glucosidase in plants infected with necrotizing viruses. Proceedings of the National Academy of Sciences of the USA 90, 8792-8796.
  • van Buuren, M., Neuhaus, J.-M., Shinshi, H., Ryals, J., and Meins, F. (1992). The structure and regulation of homeologous tobacco endochitinase genes of Nicotiana sylvestris and N. tomentosiformis origin. Mol Gen Genet 232, 460-469.
  • Sticher, L., Hofsteenge, J., Milani, A., Neuhaus, J.-M., and Meins, F., Jr. (1992). Vacuolar chitinases of tobacco: A new class of hydroxyproline-containing proteins. Science 257, 655-657.
  • Neuhaus, J.-M., Flores, S., Keefe, D., Ahl-Goy, P., and Meins, F., Jr. (1992). The function of vacuolar ß-1,3-glucanase investigated by antisense transformation. Susceptibility of transgenic Nicotiana sylvestris plants to Cercospora Nicotianae infection. Plant Molecular Biology 19, 803-813.
  • Meins, F., Jr., Neuhaus, J.-M., Sperisen, C., and Ryals, J. (1992). The primary structure of plant pathogenesis-related glucanohydrolases and their genes. In Plant Gene Research, T. Boller, ed (Wien: Springer.
  • Hart, C.M., Fischer, B., Neuhaus, J.-M., and Meins, F., Jr. (1992). Regulated inactivation of homologous gene expression in transgenic Nicotiana sylvestris plants containing a defense-related tobacco chitinase gene. Mol Gen Genet 235, 179-188.
  • De Virgilio, C., Bürckert, N., Barth, G., Neuhaus, J.-M., Boller, T., and Wiemken, A. (1992). Cloning and disruption of a gene required for growth on acetate but not on ethanol: the acetyl-coenzyme A synthetase gene of Saccharomyces cerevisiae. Yeast 8, 1043-1051.
  • Neuhaus, J.-M., Sticher, L., Meins, F., Jr., and Boller, T. (1991). A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole. Proc Natl Acad Sci USA 88, 10362-10366.
  • Neuhaus, J.-M., Ahl-Goy, P., Hinz, U., Flores, S., and Meins, F., Jr. (1991). High-level expression of a tobacco chitinase gene in Nicotiana sylvestris - Susceptibility of transgenic plants to Cercospora Nicotianae infection. Plant Mol Biol 16, 141-151.
  • Shinshi, H., Neuhaus, J.-M., Ryals, J., and Meins, F., Jr. (1990). Structure of a tobacco endochitinase gene: evidence that different chitinase genes can arise by transposition of sequences encoding a cysteine-rich domain. Plant Mol Biol 14, 357-368.
  • Shinshi, H., Wenzler, H., Neuhaus, J.-M., Felix, G., Hofsteenge, J., and Meins, F.J. (1988). Evidence for N- and C-terminal processing of a plant defense-related enzyme: Primary structure of tobacco prepro-β-1,3-glucanase. Proceedings of the National Academy of Sciences of the USA 85, 5541-5545.
  • Overath, P.O., Weigel, U., Neuhaus, J.-M., Soppa, J., Seckler, R., Riede, I., Bocklage, H., Müller-Hill, B., Aichele, G., and Wright, J.K. (1987). Lactose permease of E.coli: properties of mutants defective in substrate translocation. Proceedings of the National Academy of Sciences of the USA 84, 5535-5539.
  • Neuhaus, J.-M., Shinshi, H., Mohnen, D., and Meins, F., Jr. (1987). Characterization and regulation of tobacco chitinase genes. Journal of Cell Biochemistry 11B, 41.
  • Wright, J.K., Dornmair, K., Mitaku, S., Möröy, T., Neuhaus, J.-M., Seckler, R., Vogel, H., Weigel, U., Jähnig, F., and Overath, P.O. (1985). Lactose:H+ carrier of E.coli: kinetic mechanism, purification and structure. Ann. N. Y. Acad. Sci. 456, 326-341.
  • Wanger, M., Keiser, T., Neuhaus, J.-M., and Wegner, A. (1985). The actin treadmill (review). Can. J. Biochem. Cell Biol. 63, 414-421.
  • Neuhaus, J.-M., Soppa, J., Wright, J.K., Riede, I., Blöcker, H., Frank, R., and Overath, P.O. (1985). Properties of a mutant lactose carrier of E.coli with a Cys148 to Ser148 substitution. Federation of European Biochemical Societies Letters 185, 83-88.
  • Garavito, R.M., Hinz, U., and Neuhaus, J.-M. (1984). The crystallization of outer membrane proteins from E.coli. Studies on lamB and ompA gene products. Journal of Biological Chemistry 259, 4254-4257.
  • Neuhaus, J.-M., and Wright, J.K. (1983). Chemical modification of the lactose carrier of E.coli by plumbagin, phenylarsinoxide or diethylpyrocarbonate affects the binding of galactoside. Eur. J. Biochem. 137, 615-621.
  • Neuhaus, J.-M., Schindler, H., and Rosenbusch, J.P. (1983). The periplasmic maltose binding protein modifies the channel characteristics of maltoporin. European Molecular Biology Organisation Journal 2, 1987-1991.
  • Neuhaus, J.-M., Savko, P., Wanger, M., and Wegner, A. (1983). Kinetics of actin polymerization. In Actin: structure and function in muscle and non-muscle cells., C.G.D. Remedios and J.A. Barden, eds (Sidney: Academic Press), pp. 161-168.
  • Neuhaus, J.-M., Savko, P., Wanger, M., and Wegner, A. (1983). Treadmilling of actin (review). J Muscle Res Cell Motil 4, 507-527.
  • Neuhaus, J.-M. (1982). The receptor protein of phage l : purification, characterization and preliminary electrical studies in planar lipid bilayers. Ann. Microbiol. (Institut Pasteur) 133A, 27-32.
  • Garavito, R.M., Jenkins, J.A., Neuhaus, J.-M., Pugsley, A.P., and Rosenbusch, J.P. (1982). Structural investigations of outer membrane proteins from E. coli. Ann. Microbiol. (Institut Pasteur) 133A, 33-47.
  • Wegner, A., and Neuhaus, J.-M. (1981). Requirement of divalent cations for fast exchange of actin monomers and actin filament subunits. Journal of Molecular Biology 153, 681-693.